This compound is widely used in biochemical research as a non-hydrolyzable analog of GTP. It plays a crucial role in studying G-protein-coupled receptors and GTP-binding proteins, helping to understand their activation mechanisms. It is also employed in enzymatic assays to investigate GTPase activity and signal transduction pathways. Additionally, it is utilized in X-ray crystallography to stabilize protein structures for detailed analysis. Its stability makes it a valuable tool in exploring cellular processes involving GTP-dependent functions.