5'-GTP trisodium salt hydrate is widely used in biochemical research as a critical component in enzymatic reactions, particularly those involving kinases and GTPases. It serves as a substrate or energy source in processes like protein synthesis, signal transduction, and cellular energy metabolism. In molecular biology, it is essential for in vitro transcription and translation systems, enabling the study of gene expression and protein production. Additionally, it is utilized in assays to investigate GTP-binding proteins and their role in cellular signaling pathways. Its stability and solubility make it a reliable reagent for high-throughput screening and diagnostic applications.