6-Thioinosine 5'-Triphosphate is primarily utilized in biochemical research and molecular biology studies. It serves as a modified nucleotide analog, often incorporated into nucleic acids to study enzyme mechanisms, particularly those involving polymerases and helicases. Its thio-modified structure allows for tracking and monitoring of nucleic acid interactions and processes, making it valuable in understanding DNA and RNA dynamics. Additionally, it is used in the development of therapeutic strategies targeting nucleic acid-related pathways, such as antiviral or anticancer treatments, due to its ability to interfere with normal nucleotide incorporation and function.