ATPγS is widely used in biochemical research as a non-hydrolyzable analog of ATP, making it valuable for studying ATP-dependent processes. It is particularly useful in kinase assays, where it serves as a substrate to investigate enzyme activity without being broken down. This property allows researchers to trap and analyze intermediate states of enzymatic reactions. Additionally, ATPγS is employed in studying G-protein-coupled receptor signaling pathways, as it can activate G-proteins without subsequent deactivation. It is also used in protein phosphorylation studies to create thiophosphorylated proteins, which are resistant to phosphatases, enabling the examination of phosphorylation effects over extended periods. In structural biology, ATPγS aids in stabilizing protein complexes for X-ray crystallography or cryo-EM analysis. Its applications extend to investigating nucleotide-binding proteins, ion channels, and cellular energy metabolism.