P1,P3-Di(adenosine-5′) triphosphate ammonium salt is primarily utilized in biochemical research and enzymatic studies. It serves as a substrate in assays to investigate the activity of enzymes like adenylate kinase and other ATP-dependent enzymes. This compound is also employed in the study of nucleotide metabolism and energy transfer processes within cells. Additionally, it finds application in the development of diagnostic tools and assays for monitoring cellular energy states and enzymatic functions. Its role in mimicking natural nucleotides makes it valuable in understanding biochemical pathways and designing therapeutic agents targeting energy metabolism.