Used in biochemical research to study enzyme kinetics, particularly in reactions involving ATP-dependent enzymes, as it serves as a substrate or inhibitor in phosphorylation processes. Commonly employed in assays related to cellular energy metabolism, including those monitoring adenylate kinase and pyruvate kinase activity. Also utilized in studies of platelet aggregation, where it acts as a signaling molecule to initiate clot formation. Plays a role in in vitro diagnostic applications for assessing metabolic pathways and mitochondrial function.