Used in biochemical research to study enzyme mechanisms, particularly in kinases and phosphatases, due to the bromine substitution enhancing reactivity or binding affinity. Serves as a modified nucleotide analog in probing RNA structure and function. Employed in the development of antiviral and anticancer agents, where halogenated nucleotides can interfere with nucleic acid synthesis. Also utilized in crystallography studies to determine protein-nucleotide interactions with improved electron density from the bromine atom.