Adenosine 5'-(beta,gamma-imido)triphosphate lithium salt hydrate is widely used in biochemical research as a non-hydrolyzable analog of ATP. It is particularly valuable in studying ATP-dependent enzymes and processes, as it binds to active sites without being hydrolyzed, allowing researchers to investigate enzyme mechanisms and interactions. This compound is also utilized in structural biology, aiding in the crystallization of proteins that require ATP for stability or function. Additionally, it serves as a tool in signal transduction studies, helping to elucidate pathways involving ATP-binding proteins. Its stability and resistance to enzymatic degradation make it a reliable reagent in various in vitro assays and experimental setups.