Guanosine-5'-triphosphate disodium salt is widely used in biochemical research as a critical substrate for enzymatic reactions, particularly in studies involving RNA synthesis and protein translation. It serves as an energy source in cellular processes, making it essential for in vitro transcription and translation experiments. Additionally, it is utilized in the study of GTP-binding proteins and signaling pathways, helping to understand cellular communication and regulation. This compound is also employed in diagnostic assays and as a component in buffer systems for molecular biology applications. Its role in energy transfer and nucleotide metabolism makes it a key reagent in various biotechnological and pharmaceutical research contexts.