Guanosine 5′-triphosphate lithium salt is widely used in biochemical research as a substrate for enzymes like GTPases, which play a critical role in signal transduction and cellular processes. It is essential in studies involving protein synthesis, as it provides energy for ribosomal translation during the elongation phase. Additionally, it is utilized in nucleotide metabolism research to understand the regulation of GTP-dependent pathways. This compound is also employed in molecular biology techniques, such as in vitro transcription and RNA synthesis, where it acts as a building block for RNA polymerase. Its stability and solubility make it a preferred choice in experimental setups requiring precise GTP concentrations.