The Total Antioxidant Capacity (T-AOC) Assay Kit (FRAP) is widely used in research and clinical settings to measure the overall antioxidant potential of biological samples. It is frequently applied in studies related to oxidative stress, where it helps assess the ability of antioxidants to neutralize free radicals. This kit is particularly useful in evaluating the efficacy of dietary supplements, functional foods, and pharmaceutical products designed to boost antioxidant levels. In medical research, it aids in understanding the role of antioxidants in preventing or managing diseases linked to oxidative damage, such as cardiovascular disorders, neurodegenerative conditions, and cancer. Additionally, it is employed in environmental studies to analyze the antioxidant capacity of natural sources like plants, fruits, and vegetables, contributing to the development of antioxidant-rich products. The FRAP method is favored for its simplicity, reliability, and suitability for high-throughput analysis.