The Total Antioxidant Capacity (T-AOC) Assay Kit (TPTZ) is widely used to measure the overall antioxidant capacity in various biological samples, including serum, plasma, tissues, and cell lysates. It is particularly valuable in research focused on oxidative stress and its impact on health, as it quantifies the ability of antioxidants to neutralize free radicals. This kit is commonly applied in studies related to aging, chronic diseases, and the effects of dietary supplements or pharmaceutical compounds on antioxidant levels. It is also utilized in environmental and food science to assess the antioxidant properties of natural products, beverages, and food items. The assay provides a reliable and straightforward method for evaluating the protective effects of antioxidants against oxidative damage, making it a critical tool in both clinical and industrial research settings.