Used in biomedical research to quantify nuclear factor E2-related factor 2 (Nrf2) levels in mouse tissue samples and cell lysates. Helps assess activation of the Nrf2 antioxidant pathway, which plays a key role in cellular defense against oxidative stress and inflammation. Commonly applied in studies related to neurodegenerative diseases, liver injury, diabetes, and drug development where oxidative stress is a contributing factor. Enables researchers to evaluate the efficacy of therapeutic compounds that modulate the Nrf2 signaling pathway. Suitable for high-throughput screening and mechanistic studies in preclinical models.