Used in biomedical research to measure oxidative stress levels in biological samples such as plasma, urine, and tissue extracts. The kit enables quantification of 8-isoprostane, a stable marker formed by free radical-catalyzed peroxidation of arachidonic acid. It is widely applied in studies related to inflammation, cardiovascular diseases, pulmonary disorders, and neurodegenerative conditions. The ELISA format allows high sensitivity and specificity, making it suitable for both clinical and experimental settings where accurate assessment of lipid peroxidation is required.