Used in biomedical research to detect and quantify protein carbonyl groups in rat tissue or plasma samples, serving as a marker of oxidative stress. Widely applied in studies related to aging, neurodegenerative diseases, diabetes, and toxicology to assess cellular damage caused by reactive oxygen species. Enables high-throughput analysis in 96-well plate format, providing a sensitive and specific colorimetric measurement. Commonly used in preclinical studies to evaluate the efficacy of antioxidants or therapeutic interventions.