Used in biomedical research to measure oxidative stress levels in biological samples such as plasma, serum, urine, and tissue homogenates. The kit specifically detects 8-iso-prostaglandin F2α, a biomarker formed by free radical-induced peroxidation of arachidonic acid, independent of cyclooxygenase enzymes. It is widely applied in studies related to inflammation, cardiovascular diseases, pulmonary disorders, neurodegenerative conditions, and diabetes, where oxidative damage plays a key role. The ELISA format allows for sensitive and quantitative analysis, enabling researchers to evaluate the effectiveness of antioxidants and monitor disease progression or therapeutic interventions.