This compound is primarily utilized in biochemical research to study oxidative stress and nitration processes in proteins. It serves as a specific marker for detecting tyrosine nitration, a common modification in proteins under oxidative conditions, which is linked to various diseases such as neurodegenerative disorders, cardiovascular diseases, and inflammation. Researchers use it to mimic and investigate the effects of nitrative stress in cellular and molecular systems, helping to understand the mechanisms of oxidative damage and develop potential therapeutic strategies. Additionally, it is employed in enzymatic studies to explore the activity and inhibition of enzymes involved in tyrosine metabolism and nitration pathways.