Used in biochemical research to study lipid peroxidation and oxidative stress, it serves as a deuterated internal standard in mass spectrometry for accurate quantification of 4-hydroxynonenal (4-HNE) in biological samples. Its application aids in understanding the role of 4-HNE in diseases like cancer, neurodegenerative disorders, and cardiovascular conditions by providing precise analytical data. Additionally, it helps in evaluating the efficacy of antioxidants and therapeutic agents targeting oxidative damage.