Dimethylglyoxime is widely used as a reagent in analytical chemistry for the detection and quantification of nickel ions. It forms a bright red precipitate with nickel, making it a highly selective and sensitive method for identifying nickel in various samples, including alloys, ores, and environmental samples. Additionally, it is employed in organic synthesis as a chelating agent to stabilize metal complexes. In industrial applications, it is utilized in the purification of metals and in the preparation of catalysts. Its ability to form stable complexes with specific metals also makes it valuable in research and development for studying coordination chemistry.