EvaGreen 2X qPCR MasterMix-Low ROX is widely used in quantitative polymerase chain reaction (qPCR) applications for accurate and sensitive DNA quantification. Its primary application is in gene expression analysis, where it enables researchers to measure the levels of specific RNA transcripts. The low ROX formulation is particularly suited for instruments that require minimal passive reference dye, ensuring precise normalization of fluorescence signals across different wells and plates. This master mix is also employed in genotyping studies, where it helps detect single nucleotide polymorphisms (SNPs) and other genetic variations. Additionally, it is utilized in pathogen detection, allowing for the identification and quantification of viral or bacterial DNA in clinical and environmental samples. The EvaGreen dye binds to double-stranded DNA, providing a robust and cost-effective alternative to probe-based detection methods, making it ideal for high-throughput screening and research applications requiring high sensitivity and reproducibility.