EvaGreen 2X qPCR MasterMix-ROX is widely used in quantitative polymerase chain reaction (qPCR) applications for accurate and sensitive detection of DNA amplification. It is particularly valuable in gene expression analysis, genotyping, and mutation detection due to its ability to bind to double-stranded DNA and emit fluorescence during the PCR process. The inclusion of ROX dye ensures normalization of fluorescence signals, reducing well-to-well variability and improving data reliability. This mastermix is compatible with various real-time PCR instruments, making it versatile for research and diagnostic purposes. Its ready-to-use formulation simplifies workflow, saving time and minimizing pipetting errors. Additionally, it is suitable for high-throughput screening and multiplex assays, providing consistent and reproducible results.