EvaGreen Express 2X qPCR MasterMix-Low ROX is widely used in quantitative polymerase chain reaction (qPCR) applications for accurate and sensitive detection of nucleic acids. Its primary application is in gene expression analysis, where it enables researchers to quantify mRNA levels and study gene regulation. The low ROX formulation is particularly suited for instruments that require minimal passive reference dye, ensuring precise normalization of fluorescence signals across different wells. This mastermix is also employed in genotyping studies, allowing for the identification of genetic variations such as single nucleotide polymorphisms (SNPs) and mutations. Its high sensitivity makes it ideal for detecting low-abundance targets, which is crucial in applications like pathogen detection in clinical diagnostics or environmental monitoring. Additionally, EvaGreen Express 2X qPCR MasterMix-Low ROX is utilized in high-throughput screening workflows due to its robust performance and compatibility with various qPCR instruments. Its ease of use and reliability make it a preferred choice for researchers in fields such as molecular biology, biotechnology, and medical research.