EvaGreen miRNA qPCR Mastermix-iCycler is widely used in molecular biology for the sensitive and accurate detection of miRNA expression levels. It is particularly valuable in quantitative PCR (qPCR) experiments, where it enables the amplification and quantification of miRNA targets with high specificity. The mastermix is optimized for use with iCycler systems, ensuring reliable performance and consistent results. Its application is crucial in research areas such as cancer studies, where miRNA profiling can provide insights into disease mechanisms and potential biomarkers. Additionally, it is employed in developmental biology and stem cell research to understand gene regulation and cellular differentiation processes. The EvaGreen dye, a key component, binds to double-stranded DNA and fluoresces, allowing real-time monitoring of PCR amplification without the need for probes. This makes it a cost-effective and versatile tool for high-throughput screening and expression analysis in various biological samples.