EvaGreen miRNA qPCR MasterMix-No Dye is widely used in molecular biology for the detection and quantification of microRNAs (miRNAs) through quantitative PCR (qPCR). Its application is particularly valuable in gene expression studies, where precise measurement of miRNA levels is essential for understanding regulatory mechanisms in cellular processes. The absence of dye in this formulation allows researchers to incorporate their preferred fluorescent dyes or probes, offering flexibility in experimental design. It is commonly employed in research related to cancer, developmental biology, and other diseases where miRNA dysregulation plays a critical role. The mastermix ensures high sensitivity and specificity, making it suitable for analyzing low-abundance miRNA targets. Additionally, it is compatible with various qPCR instruments, providing versatility for different laboratory setups. Its use extends to diagnostic applications, where accurate miRNA profiling can aid in disease detection and biomarker discovery.