EvaGreen miRNA qPCR Mastermix-ROX is widely used in molecular biology for the detection and quantification of microRNAs (miRNAs) through quantitative PCR (qPCR). Its application is particularly valuable in gene expression studies, where it enables researchers to analyze miRNA levels with high sensitivity and specificity. The inclusion of ROX dye helps normalize fluorescence variations across wells, ensuring accurate and reproducible results. This mastermix is compatible with various qPCR instruments, making it versatile for use in diagnostic research, biomarker discovery, and studies of miRNA involvement in diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions. Its optimized formulation allows for efficient amplification of miRNA targets, even in low-abundance samples, making it a reliable tool for advancing miRNA research and clinical applications.