PDK1-IN-RS2 is primarily utilized in biomedical research, particularly in the study of cellular signaling pathways. It acts as an inhibitor of PDK1 (3-phosphoinositide-dependent protein kinase 1), which plays a crucial role in the PI3K/AKT/mTOR pathway. This pathway is essential for regulating cell growth, survival, and metabolism, making PDK1-IN-RS2 a valuable tool for investigating cancer biology and other diseases linked to dysregulated signaling. Researchers use this compound to explore potential therapeutic strategies targeting PDK1, aiming to develop treatments for cancers and metabolic disorders. Its application extends to in vitro and in vivo studies, helping to elucidate the molecular mechanisms underlying disease progression and resistance to therapies.