1-Hydroxybenzotriazole is widely used as an additive in peptide synthesis to enhance coupling efficiency and reduce racemization. It acts as a catalyst in the formation of amide bonds, making it essential in the production of peptides and proteins in pharmaceutical and biochemical research. Additionally, it is employed in organic synthesis to facilitate esterification and other condensation reactions, improving yields and reaction rates. Its role in preventing side reactions and improving selectivity makes it valuable in the development of complex organic molecules.