Widely used in molecular and cellular biology research, this peptide tag enables efficient detection and purification of recombinant proteins. It is commonly employed in immunoassays such as Western blotting, immunoprecipitation, and immunofluorescence due to its high affinity for anti-Flag antibodies. The trifluoroacetate salt form ensures solubility and stability in aqueous solutions, making it suitable for in vitro and in vivo applications. Researchers often fuse the peptide to target proteins during expression to facilitate tracking, isolation, and localization without significantly altering the protein’s native function. Its small size minimizes interference with protein structure, allowing reliable experimental outcomes in protein interaction studies and functional assays.