Used as a pH indicator in various laboratory applications, particularly in titration experiments to determine the acidity or alkalinity of solutions. It changes color from yellow (pH 3.0) to blue (pH 4.6), making it useful for detecting endpoints in acid-base reactions. Also employed in gel electrophoresis to track the progress of DNA or protein separation, as it migrates similarly to small DNA fragments. Additionally, it serves as a staining agent in microscopy to enhance the visibility of cellular structures. Its water-soluble nature makes it convenient for use in aqueous solutions.