Pregna-4,6-diene-3,20-dione is primarily used in pharmaceutical research and development, particularly in the synthesis of steroid-based compounds. It serves as a key intermediate in the production of various hormonal drugs, including corticosteroids and progestogens. These drugs are essential for treating conditions such as inflammation, autoimmune disorders, and hormonal imbalances. Additionally, its unique structure makes it valuable in studying steroid metabolism and developing novel therapeutic agents. In the field of organic chemistry, it is also utilized as a building block for creating complex molecules with potential biological activity.