Methoxycarbonyl-L-tert-leucine is widely used in the synthesis of peptides and pharmaceutical compounds. It serves as a key intermediate in the production of protease inhibitors, which are essential in treating diseases like HIV and hepatitis C. Its unique structure enhances the stability and bioavailability of peptide-based drugs. Additionally, it is utilized in the development of chiral molecules for asymmetric synthesis, playing a critical role in creating enantiomerically pure substances for therapeutic applications. Its application also extends to research in biochemistry and medicinal chemistry, where it aids in studying enzyme mechanisms and designing novel drug candidates.