E7449 is a potent and selective dual PARP1/Tankyrase 1/2 (TNKS1/2) inhibitor (IC₅₀s = 2.51 nM, 0.23 nM, 0.92 nM, respectively). It is primarily used in pharmaceutical research and development for studying DNA damage response and repair pathways, particularly in oncology. Employed in biochemical assays, cell-based studies, and preclinical research to investigate mechanisms of action, drug efficacy, and potential anticancer activity.