UV-VIS Total Phenolic Content (Folin-Ciocalteu)

  • Product Code: 30976
Spectrophotometric method measuring total phenolics in plant extracts via reaction with Folin-Ciocalteu reagent, absorbance at 765 nm, expressed as gallic acid equivalents.
฿2,990.00

label Sample Names

info Please enter each sample name you want included for testing

assignment Instructions

info Please include any specific requirements or conditions for testing

description Sample Requirement

plant extract (lyophilized or dry) · 0.1 · grams

insert_drive_file Report of Previously Tested Sample

Select a tab and click View to open the report.

timeline Service Steps

Step Procedure Expected Result
1
Prepare gallic...
check_circle_outline Clear, colorless solutions.
2

Dissolve 10...

check_circle_outline

Clear to slightly hazy solution; no undissolved particles.

3

The test...

check_circle_outline

reaction mixture

4

After 5...

check_circle_outline

development of blue color

5

check_circle_outline

absorbance of reaction

6

check_circle_outline

sample concentration (mg GAE/ g extract)

UV-VIS Total Phenolic Content (Folin-Ciocalteu)

Analysis of Total Phenolic Content using Folin-Ciocalteu method

UV-VIS Total Phenolic Content (Folin-Ciocalteu, 1 sample)

 

Analysis of Total Phenolic Content using Folin-Ciocalteu method

 

Measuring the total phenolic content of a sample using the Folin-Ciocalteu assay with UV-Vis spectrophotometry is a common method for quantifying the concentration of phenolic compounds, which are natural antioxidants found in many plants and foods. Here's a general protocol for performing a UV-Vis total phenolic content assay using the Folin-Ciocalteu reagent

Mechanism -
Appearance -
Longevity -
Strength -
Storage -
Shelf Life -
Allergen(s) -
Dosage (Range) -
Dosage (Per Day) -
Mix Method -
Heat Resistance -
Stable in pH range -
Solubility -
Product Types -
INCI -
Service Steps
Step Procedure Expected Result
1 Prepare gallic... Clear, colorless solutions.
2

Dissolve 10...

Clear to slightly hazy solution; no undissolved particles.

3

The test...

reaction mixture

4

After 5...

development of blue color

5

sample concentration (mg GAE/ g extract)