UV-VIS Total Flavonoids Content (Aluminum Chloride Colorimetric Assay)
- Product Code: 30977
UV-Vis spectrophotometric assay for total flavonoid content in plant extracts via complexation with aluminum chloride, quantified as quercetin equivalents at 420 nm.
description Sample Requirement
dry plant extract · 10 · mg
insert_drive_file Report of Previously Tested Sample
timeline Service Steps
| Step | Procedure | Expected Result |
|---|---|---|
|
1
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Weigh 10... |
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Clear solution; if insoluble, sonicate or heat gently <40°C. |
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2
|
Prepare quercetin... |
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Clear standards ready for assay.
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3
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Into labeled... |
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sample |
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4
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Add 1.8... |
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sample and AlCl3 mixture |
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5
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After mixing,... |
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yellow color development |
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6
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The absorbance... |
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absorbance of reaction |
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7
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The flavonoid... |
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flavonoid content |
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