Ro 90-7501 is primarily used in research settings as a potent and selective inhibitor of the protein phosphatase 2A (PP2A). Its application is significant in studying cellular signaling pathways, particularly those involving PP2A, which plays a crucial role in regulating various cellular processes such as cell growth, division, and apoptosis. Researchers utilize Ro 90-7501 to investigate the effects of PP2A inhibition on cancer cells, as PP2A is often implicated in tumor suppression. By inhibiting PP2A, scientists can better understand its role in cancer progression and potentially identify new therapeutic targets. Additionally, Ro 90-7501 is used in neuroscience research to explore the involvement of PP2A in neurodegenerative diseases, where dysregulation of this enzyme may contribute to pathological conditions. Overall, Ro 90-7501 serves as a valuable tool in biochemical and pharmacological studies aimed at elucidating the complex roles of PP2A in health and disease.