(E/Z)-BCI hydrochloride is primarily utilized in biochemical research, particularly in the study of cellular signaling pathways. It serves as a potent and selective inhibitor of dual-specificity phosphatase 6 (DUSP6), an enzyme involved in regulating the ERK/MAPK signaling pathway. By inhibiting DUSP6, this compound helps researchers investigate the role of ERK signaling in various cellular processes, including cell proliferation, differentiation, and survival. Its application is significant in cancer research, as dysregulation of the ERK pathway is often associated with tumor growth and progression. Additionally, it is used in studies aiming to understand the molecular mechanisms underlying diseases linked to MAPK pathway abnormalities.