VO-Ohpic trihydrate is a potent and selective inhibitor of the PTEN (phosphatase and tensin homolog) phosphatase enzyme. It is widely used in biochemical and cellular research to modulate the PI3K/Akt signaling pathway, which plays a critical role in cell survival, proliferation, and metabolism. By inhibiting PTEN, VO-Ohpic trihydrate enhances phosphorylation of Akt, making it a valuable tool for studying insulin signaling, cancer progression, and metabolic disorders. It is commonly applied in studies involving neuronal survival, where activation of the PI3K pathway can protect against apoptosis. Researchers also use it to investigate the role of PTEN in stem cell differentiation and tissue regeneration. Due to its cell permeability and stability, VO-Ohpic trihydrate is suitable for both in vitro and cell-based assays, allowing for precise control over PTEN activity in experimental models.