Used primarily in biochemical research as a substrate for phosphatase enzymes, enabling the study of enzyme activity and kinetics. It is also employed in histochemistry for detecting alkaline phosphatase in tissue samples, aiding in the identification of specific cellular components. Additionally, it serves as a reagent in diagnostic assays to measure enzyme levels in clinical samples, contributing to the diagnosis of various medical conditions. Its role in enzymatic studies makes it valuable in both academic and clinical laboratories.