Used primarily in biochemical research, this compound serves as a versatile tool for photoaffinity labeling. Researchers employ it to study protein-ligand interactions, enabling the identification and mapping of binding sites on proteins. Its diazirine group, upon exposure to ultraviolet light, generates highly reactive carbenes that form covalent bonds with nearby molecules, making it invaluable for capturing transient interactions. Additionally, it aids in the development of probes for understanding molecular mechanisms in biological systems, contributing to drug discovery and the study of enzyme functions.