This compound is primarily utilized in photoaffinity labeling studies, a technique employed in biochemistry and molecular biology to investigate protein-ligand interactions. The diazirine group within the molecule can be activated by UV light, generating a highly reactive carbene intermediate. This carbene can form covalent bonds with nearby molecules, allowing researchers to "tag" and identify binding sites on proteins or other biomolecules. Its trifluoromethyl group enhances stability and reactivity, making it a valuable tool for probing complex biological systems. Applications include drug discovery, where it helps map drug-target interactions, and structural biology, aiding in the elucidation of protein structures and functions.