This compound is primarily used in biochemical research as a photoactivatable crosslinker. It is designed to study protein-protein interactions and other molecular binding events. Upon exposure to UV light, the diazirine group generates a reactive carbene intermediate, which can form covalent bonds with nearby molecules, effectively "capturing" transient interactions. This makes it a valuable tool for mapping protein interaction networks and understanding molecular mechanisms in complex biological systems. Additionally, it is utilized in proteomics to identify binding partners of specific proteins or to investigate the spatial organization of proteins within cells. Its incorporation into peptides or proteins allows for precise targeting and labeling in live-cell imaging and structural biology studies.