Cis-aconitic acid is primarily utilized in biochemical research and industrial applications. In biochemistry, it serves as an intermediate in the tricarboxylic acid (TCA) cycle, playing a crucial role in cellular energy production. Researchers study its behavior to understand metabolic pathways and enzyme functions. Industrially, it is used in the production of biodegradable plastics and as a chelating agent in metal processing. Additionally, it finds application in the synthesis of certain pharmaceuticals and as a precursor in organic chemistry reactions. Its ability to form stable complexes with metals makes it valuable in water treatment processes to control metal ion concentrations.