PI-103 Hydrochloride is primarily used in biochemical research as a potent inhibitor of PI3K (phosphoinositide 3-kinase) and mTOR (mammalian target of rapamycin) signaling pathways. It is particularly valuable in studies focused on understanding cancer cell proliferation, survival, and metabolism, as these pathways are often dysregulated in tumors. Researchers use it to investigate the effects of inhibiting these pathways in various cancer models, aiding in the development of targeted therapies. Additionally, it serves as a tool compound in preclinical studies to explore the role of PI3K and mTOR in other diseases, such as diabetes and neurodegenerative disorders. Its ability to simultaneously target both pathways makes it a versatile agent for studying complex cellular signaling networks.