VS-5584 is primarily utilized in cancer research due to its potent inhibitory effects on PI3K and mTOR signaling pathways. These pathways are crucial for cell growth, proliferation, and survival, making VS-5584 a valuable tool in studying tumor biology and developing targeted cancer therapies. It is often used in preclinical studies to evaluate its efficacy in suppressing cancer cell growth and inducing apoptosis, particularly in cancers with dysregulated PI3K/mTOR signaling. Additionally, VS-5584 helps researchers understand the mechanisms of resistance to PI3K/mTOR inhibitors, aiding in the development of more effective treatment strategies. Its application extends to investigating combination therapies, where it is tested alongside other anticancer agents to enhance therapeutic outcomes.