PKI 14-22 amide, myristoylated, is primarily used in biochemical research to study protein kinase A (PKA) signaling pathways. It acts as a potent and specific inhibitor of PKA, making it valuable for understanding the role of PKA in cellular processes such as metabolism, gene expression, and cell division. Researchers utilize this compound to dissect the mechanisms of PKA-dependent phosphorylation events and their impact on various physiological and pathological conditions. Its myristoylation enhances membrane association, allowing for targeted inhibition of PKA activity in specific cellular compartments. This compound is particularly useful in studies involving heart function, neuronal signaling, and hormonal regulation, where PKA plays a critical role.