Phorbol 12,13-dibutyrate is widely used in biomedical research as a potent activator of protein kinase C (PKC), a family of enzymes involved in various cellular processes. It is commonly employed in studies to investigate signal transduction pathways, particularly those related to cell proliferation, differentiation, and apoptosis. Researchers use it to induce and analyze PKC-mediated responses in cell cultures, helping to understand the role of PKC in diseases such as cancer and inflammation. Additionally, it serves as a tool in pharmacological studies to test the efficacy of PKC inhibitors. Its ability to mimic diacylglycerol, a natural PKC activator, makes it valuable for exploring cellular mechanisms and developing therapeutic strategies.