Hygromycin B is widely used as a selective agent in cell culture and molecular biology applications. It is commonly employed to select and maintain cells that have been genetically modified with resistance genes, particularly in mammalian, bacterial, and yeast cell systems. The compound inhibits protein synthesis in sensitive cells by binding to the 30S subunit of the bacterial ribosome, making it effective for eliminating non-resistant cells during stable transfection experiments. In research settings, Hygromycin B standard solution is used for creating selection pressure in media to isolate clones expressing the hygromycin resistance gene (such as the hph gene). This makes it invaluable in gene knockout studies, transgenic cell line development, and recombinant protein production. Its use as a standard solution ensures accurate and consistent dosing, which is critical for reproducible selection conditions. Additionally, due to its well-characterized mode of action and stability, Hygromycin B is used in microbiology and biotechnology for quality control and validation of resistance markers in genetically engineered organisms.