Macerozyme R-10 is widely used in plant biology for the enzymatic digestion of plant cell walls. It is particularly effective in isolating protoplasts from plant tissues, which are then used in various research applications such as genetic engineering, cell fusion, and the study of cell wall regeneration. The enzyme mixture helps break down pectin and other polysaccharides, facilitating the release of intact protoplasts. This makes it a valuable tool in plant biotechnology for creating transgenic plants, studying cell physiology, and developing somatic hybrids. Additionally, it is employed in tissue culture to enhance the efficiency of cell separation and regeneration processes.