G 418 disulfate salt is widely used in molecular biology and genetic research as a selective agent. It is commonly employed to select and maintain eukaryotic cells that have been genetically modified to express a resistance gene, typically the neomycin resistance gene (neoR). This chemical is particularly useful in cell culture experiments where researchers need to ensure that only the cells containing the desired genetic modification survive and proliferate. It is also applied in the creation of stable cell lines, allowing scientists to study gene function and expression in a controlled environment. Additionally, G 418 disulfate salt is utilized in the development of transgenic organisms, aiding in the identification and selection of successfully engineered specimens. Its effectiveness in inhibiting protein synthesis in non-resistant cells makes it a valuable tool for genetic selection and research applications.