Used primarily in molecular biology for the selection and maintenance of eukaryotic cells transfected with a hygromycin resistance gene. It is effective in eliminating non-transfected cells, ensuring that only those with the desired genetic modification survive. Commonly applied in research involving mammalian, plant, and bacterial cell cultures to study gene function, protein expression, and cellular processes. It is also utilized in the development of genetically modified organisms (GMOs) and in biopharmaceutical production to select stable cell lines producing therapeutic proteins.